Anti-tumor protease preparations

PAT 00-00-89 04844897 NDN- 167-0148-4895-2

INVENTOR(S)- Maeda, Hiroshi; Matsumura, Yasuhiro; Asami, Osamu; Tanaka,
Hideyuki; Sasaki, Ikuharu

PATENT NUMBER- 04844897
PATENT APPLICATION NUMBER- 906240
DATE FILED- 1986-09-12
PATENT DATE- 1989-07-04
NUMBER OF CLAIMS- 4
EXEMPLARY CLAIMS- 1
FIGURES- 5
ART/GROUP UNIT- 185
PATENT CLASS- Invention (utility) patent
INVENTOR COUNTRY/ZIPCODE- JPX; JPX; JPX; JPX; JPX
PATENT ASSIGNEE(S)- Maeda, Hiroshi; Amano Pharmaceutical Co., Ltd.
ASSIGNEE CITY- Kumamoto; Nagoya
ASSIGNEE COUNTRY- JPX; JPX
ATTORNEY, AGENT, OR FIRM- Wegner & Bretschneider
U.S. PATENT CLASS- 424094300O
U.S. CLASSIFICATION REFS.- X424094630; X435220000; X435221000
INTERNATIONAL PATENT CLASS- A61K03754; C12N00952; C12N00954
PATENT REFERENCE(S)- 3823072; 4066503; 4079125; 4495285; 4514388
PATENT REFERENCED BY- 05856451; 06030933; 06322815
PATENT APPLICATION PRIORITY- 60-201607; 61-184126
PRIORITY COUNTRY CODE- JPX; JPX
PRIORITY DATE- 19850913; 19860807

Method of treating a tumor in a mammal comprises administering to said
mammal an effective anti-tumor amount of proteases originating from
microorganisms. ; Method of treating a tumor in a mammal comprises
administering to said mammal an effective anti-tumor amount of a protease
originating from a microorganism which protease is chemically modified by
one of the following procedures: ; (a) coupling with a saccharide, ; (b)
introduction of a hydrophobic polymeric group, ; (c) alteration of electric
charge of the protein surface, ; (d) conjugation with a low molecular weight
anti-tumor agent of molecular weight less than 2,000, ; (e) formation of
dimer or oligomer by cross-linking of protease molecules, ; (f) conjugation
with a synthetic polycation, ; (g) conjugation with a synthetic polyanion,
and ; (h) combination of the above-mentioned procedures. ; Microorganism
protease is chemically modified by one of the following procedures: ; (a)
coupling with a saccharide, ; (b) introduction of a hydrophobic polymeric
group, ; (c) alteration of electric charge of the protein surface, ; (d)
conjugation with a low molecular weight anti-tumor agent of molecular weight
less than 2,000, ; (e) formation of dimer or oligomer by cross-linking of
protease molecules, ; (f) conjugation with a synthetic polycation, ; (g)
conjugation with a synthetic polyanion, and ; (h) combination of the
above-mentioned procedures.
EXEMPLARY CLAIMS- Claim- 1. Method for treating a tumor in a mammal which
comprises directly administering into the tumor of said mammal an effective
anti-tumor amount of proteases produced from a source, said source selected
from the group consisting of Serratia marcescens, Bacillus sp. and
Streptomyces griseus, wherein said protease is other than an acid protease .